Molecular Biology and Evolution, Vol 13, 903-917, Copyright © 1996 by Society for Molecular Biology and Evolution
DS Hibbett
Group I introns were discovered inserted at the same position in the
nuclear small-subunit ribosomal DNA (nuc-ssu-rDNA) in several species of
homobasidiomycetes (mushroom-forming fungi). Based on conserved intron
sequences, a pair of intron-specific primers was designed for PCR
amplification and sequencing of intron-containing rDNA repeats. Using the
intron-specific primers together with flanking rDNA primers, a PCR assay
was conducted to determine presence or absence of introns in 39 species of
homobasidiomycetes. Introns were confined to the genera Panellus,
Clavicorona, and Lentinellus. Phylogenetic analyses of nuc-ssu-rDNA and
mitochondrial ssu-rDNA sequences suggest that Clavicorona and Lentinellus
are closely related, but that Panellus is not closely related to these. The
simplest explanation for the distribution of the introns is that they have
been twice independently gained via horizontal transmission, once on the
lineage leading to Panellus, and once on the lineage leading to Lentinellus
and Clavicorona. BLAST searches using the introns from Panellus and
Lentinellus as query sequences retrieved 16 other similar group I introns
of nuc-ssu-rDNA and nuclear large-subunit rDNA (nuc-lsu-rDNA) from fungal
and green algal hosts. Phylogenetic analyses of intron sequences suggest
that the mushroom introns are monophyletic, and are nested within a clade
that contains four other introns that insert at the same position as the
mushroom introns, two from different groups of fungi and two from green
algae. The distribution of host lineages and insertion sites among the
introns suggests that horizontal and vertical transmission, homing, and
transposition have been factors in intron evolution. As distinctive,
heritable features of nuclear rDNAs in certain lineages, group I introns
have promise as phylogenetic markers. Nevertheless, the possibility of
horizontal transmission and homing also suggest that their use poses
certain pitfalls.
ORIGINAL ARTICLE
Phylogenetic evidence for horizontal transmission of group I introns in the nuclear ribosomal DNA of mushroom-forming fungi
Harvard University Herbaria, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138, USA. dhibbett@oeb.harvard.edu
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K. J. Martin Introduction to Molecular Analysis of Ectomycorrhizal Communities Soil Sci. Soc. Am. J., March 12, 2007; 71(2): 601 - 610. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Binder, D. S. Hibbett, Z. Wang, and W. F. Farnham Evolutionary relationships of Mycaureola dilseae (Agaricales), a basidiomycete pathogen of a subtidal rhodophyte Am. J. Botany, April 1, 2006; 93(4): 547 - 556. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q.-M. Zhou, S.-Y. Guo, M.-R. Huang, and J.-C. Wei A study of the genetic variability of Rhizoplaca chrysoleuca using DNA sequences and secondary metabolic substances. Mycologia, January 1, 2006; 98(1): 57 - 67. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Wang, M. Binder, and D. S. Hibbett Life history and systematics of the aquatic discomycete Mitrula (Helotiales, Ascomycota) based on cultural, morphological, and molecular studies Am. J. Botany, September 1, 2005; 92(9): 1565 - 1574. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Wang, M. Binder, Y.-C. Dai, and D. S. Hibbett Phylogenetic relationships of Sparassis inferred from nuclear and mitochondrial ribosomal DNA and RNA polymerase sequences Mycologia, September 1, 2004; 96(5): 1015 - 1029. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. HAUGEN, H. J. RUNGE, and D. BHATTACHARYA Long-term evolution of the S788 fungal nuclear small subunit rRNA group I introns RNA, July 1, 2004; 10(7): 1084 - 1096. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Haugen and D. Bhattacharya The spread of LAGLIDADG homing endonuclease genes in rDNA Nucleic Acids Res., April 6, 2004; 32(6): 2049 - 2057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-J. Cote, M. Prud'homme, A. J. Meldrum, and M.-C. Tardif Variations in sequence and occurrence of SSU rDNA group I introns in Monilinia fructicola isolates Mycologia, March 1, 2004; 96(2): 240 - 248. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Haugen, V. Reeb, F. Lutzoni, and D. Bhattacharya The Evolution of Homing Endonuclease Genes and Group I Introns in Nuclear rDNA Mol. Biol. Evol., January 1, 2004; 21(1): 129 - 140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. B. Lickey, K. W. Hughes, and R. H. Petersen Variability and Phylogenetic Incongruence of an SSU nrDNA Group I Intron in Artomyces, Auriscalpium, and Lentinellus (Auriscalpiaceae: Homobasidiomycetes) Mol. Biol. Evol., November 1, 2003; 20(11): 1909 - 1916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. C. Adams, R. S. Surve-Iyer, and A. F. Iezzoni Ribosomal DNA sequence divergence and group I introns within the Leucostoma species L. cinctum, L. persoonii, and L. parapersoonii sp. nov., ascomycetes that cause Cytospora canker of fruit trees Mycologia, November 1, 2002; 94(6): 947 - 967. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. B. Lickey, K. W. Hughes, and R. H. Petersen Biogeographical patterns in Artomyces pyxidatus Mycologia, May 1, 2002; 94(3): 461 - 471. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Nikoh and T. Fukatsu Evolutionary Dynamics of Multiple Group I Introns in Nuclear Ribosomal RNA Genes of Endoparasitic Fungi of the Genus Cordyceps Mol. Biol. Evol., September 1, 2001; 18(9): 1631 - 1642. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Perotto, P. Nepote-Fus, L. Saletta, C. Bandi, and J. P. W. Young A Diverse Population of Introns in the Nuclear Ribosomal Genes of Ericoid Mycorrhizal Fungi Includes Elements with Sequence Similarity to Endonuclease-Coding Genes Mol. Biol. Evol., January 1, 2000; 17(1): 44 - 59. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Cho, Y.-L. Qiu, P. Kuhlman, and J. D. Palmer Explosive invasion of plant mitochondria by a group I intron PNAS, November 24, 1998; 95(24): 14244 - 14249. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Prade, J. Griffith, K. Kochut, J. Arnold, and W. E. Timberlake In vitro reconstruction of the Aspergillus (= Emericella) nidulans genome PNAS, December 23, 1997; 94(26): 14564 - 14569. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Hibbett, E. M. Pine, E. Langer, G. Langer, and M. J. Donoghue Evolution of gilled mushrooms and puffballs inferred from ribosomal DNA sequences PNAS, October 28, 1997; 94(22): 12002 - 12006. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Huelsenbeck and B. Rannala Phylogenetic Methods Come of Age: Testing Hypotheses in an Evolutionary Context Science, April 11, 1997; 276(5310): 227 - 232. [Abstract] [Full Text]
|
|